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marathonrt  (RNAConnect Inc)


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    Structured Review

    RNAConnect Inc marathonrt
    Marathonrt, supplied by RNAConnect Inc, used in various techniques. Bioz Stars score: 95/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/marathonrt/MarathonRT/custom%40200035%4042755360
    Average 95 stars, based on 144 article reviews
    marathonrt - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    In Vitro:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Incubation:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Amplification:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    cDNA Synthesis:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Polymerase Chain Reaction:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Quantitative RT-PCR:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Sequencing:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Modification:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Extraction:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Amplicon Sequencing:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Purification:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    RNA Modification:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo

    Synthesized:

    Article Title: Interdependent RNA structural motifs at the 3'-terminus of the West Nile virus genome regulate viral growth.
    Article Snippet: e mixed with 2 pmol of gene-specific primers ( Supplementary Table S2 ). Primers were annealed to RNAs by heating to 90◦C for 1 min and 30◦C for 2 min. The RNAs were then reverse transcribed (RT) with MarathonRT (RNAConnect), 2X MarathonRT buffer, (100 mM Tris–HCl pH 8.3, 400 mM KCl, 4 mM MgCl2 , 10 mM DTT, and 40% glycerol) and 0.5 mM dNTP (NEB, cat# N0447S). RT reactions were incubated at 42◦C fo



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